Composite
Pre B1

Part:BBa_K4060104

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)


[Pre B1] Pre A1 ligated before TetR

The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None